In April 1863, the Trustees of the Congregation of The Trinity Presbyterian Church purchased a lot on Waterloo Avenue in Reeseville (later Berwyn) for $150 to erect a church and establish a cemetery. The cemetery was subdivided into 142 numbered lots.

The last person was interred in the cemetery in 1924. By 1929, it had become unsightly and unsuitable for the repose of the dead, and a decree was issued to vacate the cemetery in January of that year. The church purchased a lot in the Great Valley Presbyterian Church Cemetery for the purpose of moving the remains of Trinity's cemetery to a more suitable location. Efforts continued into the 1930s to locate relatives of those still interred in the Trinity churchyard. Some families moved their relatives to other burying places, including Old Saint David's Church in Radnor, though no action was officially taken to disestablish the cemetery.

In October of 1951, the bodies still interred in the Berwyn cemetery were exhumed and removed to the Trinity Lot in the Great Valley Presbyterian cemetery purchased earlier for that purpose.

The transfer of the remains did not have the exactness one would have preferred. In the earliest years, simple wooden coffins had been used for burial. Removal was difficult. The only grave chart in Trinity's records was made in 1906 and was incomplete, even at that time. Many graves had no identification at all.

Identified remains were carefully marked at the removal site and in the records of Great Valley Presbyterian cemetery. These remains were placed in small wooden boxes. A large box was purchased for the unidentified remains. All of the gravestones, sans one removed earlier to the Great Valley Presbyterian Church Cemetery, were broken and buried under Trinity's parking lot in this removal.

Today, a church expansion occupies the site.