Infante Luís of Portugal, Duke of Beja (3 March 1506, in Abrantes – 27 November 1555, in Marvila, Lisbon) was the second son of King Manuel I of Portugal and his second wife Maria of Aragon (the third daughter of the Catholic Monarchs). He participated in the Conquest of Tunis. Luís succeeded his father as the Duke of Beja and was also made Constable of the Kingdom (Portuguese: Condestável do Reino) and Prior of the Order of Saint John of Jerusalem, with its Portuguese headquarters in the town of Crato.
In the Conquest of Tunis (1535) Luís, brother-in-law of Charles V, commanded the Portuguese army. The Portuguese galleon São João Baptista, also known as Botafogo was specifically requested by Charles V, and it was the most powerful ship in the world at the time, with 366 bronze cannons. It was the Botafogo spur ram that broke up the chains at La Goletta, which defended the port entrance of Tunis, allowing the Christian allied fleet to reach and conquer the city.
He did not marry but had a natural son by Violante Gomes, a Pelicana (the she-pelican), a New Christian, who is said to have died a Nun in Almoster, Santarém, on 16 July 1568, daughter of Pedro Gomes, from Évora. Some say they eventually married perhaps at Évora, thus legitimating their issue for every purpose. Their son António, Prior of Crato, would be one of the claimants to the throne after the death of King Sebastian of Portugal in the disastrous Battle of Alcácer-Quibir and the subsequent dynastic crisis that followed, and, according to some historians, the King of Portugal for approximately a month in the year 1580.
Isabella of Portugal
Beatriz of Portugal
Fernando of Portugal
Afonso of Portugal
Duarte of Portugal
Antonio of Portugal